Workshop

Workshop 1 topic : Cell culture techniques
This is 1-day hands-on workshop on the following techniques using mice embryonic cell line and human cancer cell lines.

What is cell culture?
Cell culture refers to the removal of cells from an animal or plant and their subsequent growth in a favorable artificial environment. The cells may be removed from the tissue directly and disaggregated by enzymatic or mechanical means before cultivation, or they may be derived from a cell line or cell strain that has already been already established.
Workshop objective:
The workshop provides a solid basis for the understanding of the growth of animal cells and combines lecture and laboratory exercises aimed at giving participants a deeper understanding of serum-free media applications and related topics.
The workshop is designed to provide a solid understanding of cell culture through classroom presentation and laboratory work. Clients will gain significant experience in the manipulation of animal cells through culture. Through integrated learning methods, utilizing hands-on training to reinforce lecture material participants will learn the biological basis and relevance of the process of culturing cells. This will allow clients to translate the techniques into applications in their own laboratories. At the end of the workshop we will conduct a full workshop review, and data troubleshootig session. This
workshop provide prerequisite training for the culture of numerous cell types including stem cells.
Topics include:

Introduction to cell and tissue culture, serum containing media and the use of serum. cell counting and viability determination, development of growth curves, growth of adherent and suspension cells, transfection technologies for recombinant protein expression in animal cells, cryopreservation and cryothawing of animal cells, cell cloning and primary cell culture.
Our Visiting Professor who is expert at Cell culture Techniques, will share her expertise in carrying out cell culture and functional assays with the students in Kazakhstan through this workshop.

Focus of the workshop:
 Basic cell culture techniques,
o Introducing cell culture equipment and biosafety rules
o Sub culturing and maintenance of cell lines
o Collection of cells for DNA and protein isolation
o Thawing and freezing techniques
o Observation cell lines under inverted microscopy
 Media preparation
 Applications of cell culture in clinics and research

 

The price of this workshop: 10000 tenge

Deadline for registration to workshop: March 10th, 2018


Worshop 2 Title : Using Genomic Databases and Designing Primer for PCR Experiment

Description:

Advancements in the field of Molecular Biology and Genetics gave rise to a new field that is called Bioinformatics. Currently bioinformatics is a rapidly growing field that takes its roots from many other fields like molecular biology and genetics, statistics, and computer science. With huge amount of biological data, researchers try to find new ways to analyze and understand them and reveal similarities and differences between DNA, RNA and Protein sequences. 


During the workshop we are going to learn about genomic databases like NCBI-GenBank, UNIPROT, EMBL and understand sequence annotations that helps differenciate between sequences. In addition we are going learn, how to design a primers for very important experiment in Molecular Biology and Genetics that is called PCR (Polymerase Chain Reaction) with several tools and later check the validity of the designed primers by using web applications and help of state of the art algorithms i.e., global and local alignment algorithms.
Registration fee for this workshop: 7500 tenge
Deadline for registration for this workshop: March 30, 2018