Workshop topic: Cell culture techniques
This is 1-day hands-on workshop on the following techniques using human/mice embryonic cell line and cancer cell lines.
What is cell culture?
Cell culture refers to the removal of cells from an animal or plant and their subsequent growth in a favorable artificial environment. The cells may be removed from the tissue directly and disaggregated by enzymatic or mechanical means before cultivation, or they may be derived from a cell line or cell strain that has already been already established.
The workshop provides a solid basis for the understanding of the growth of animal cells and combines lecture and laboratory exercises aimed at giving participants a deeper understanding of serum-free media applications and related topics.
The workshop is designed to provide a solid understanding of cell culture through classroom presentation and laboratory work. Clients will gain significant experience in the manipulation of animal cells through culture. Through integrated learning methods, utilizing hands-on training to reinforce lecture material participants will learn the biological basis and relevance of the process of culturing cells. This will allow clients to translate the techniques into applications in their own laboratories. At the end of the workshop we will conduct a full workshop review, and data troubleshooting session. This workshop provide prerequisite training for the culture of numerous cell types including stem cells.
Topics include: Introduction to cell and tissue culture, serum containing media and the use of serum. cell counting and viability determination, development of growth curves, growth of adherent and suspension cells, transfection technologies for recombinant protein expression in animal cells, cryopreservation and cryothawing of animal cells, cell cloning and primary cell culture.
Our Visiting Professor who is expert at Cell culture Techniques, will share her expertise in carrying out cell culture and functional assays with the students in Kazakhstan through this workshop.
Focus of the workshop:
- Basic cell culture techniques,
- Introducing cell culture equipment and biosafety rules
- Sub culturing and maintenance of cell lines
- Collection of cells for DNA and protein isolation
- Thawing and freezing techniques
- Observation cell lines under inverted microscopy
- Media preparation
- Applications of cell culture in clinics and research